Abstract: In this study, pathological, histopathological findings and slaughter judgment of a case of eosinophilic myositis in a mixed breed cow are described. In post mortem inspection, multifocal large irregular areas of greenish discoloration of various groups of skeletal muscles were seen. Representative samples of affected muscles were placed in 10% neutral buffered formalin, embedded in paraffin, sectioned at 4-6 μm and stained routinely with hematoxiline eosin. Microscopically, examinations demonstrate that affected areas are severely infiltrated by a large number of eosinophils, fewer macrophages and lymphocytes.