Abstract: Eggs of laying hens were used to study residue depletion of Florfenicol (FF) and its metabolite Florfenicol Amine (FFA) after oral dose (25.0, 50.0 and 100.0 mg kg-1 body weight), once daily for 5 days. The residues were determined by High Performance Liquid Chromatography with Fluorescence Detection (HPLC-FLD). The maximum concentration of FF was all detected at 1 day in the albumen, yolk and whole eggs; the maximum concentration of FFA was detected at 1 day in the albumen and whole eggs, at 3 days in the yolk after cessation of medication. After oral administration of 100.0 mg kg-1, concentrations of FF and FFA were all below the LODs (1.5 μg kg-1 for FF and 0.5 μg kg-1 for FFA) at 7 days for the albumen, 11 days for yolk and 10 days for whole egg after cessation of medication.