Abstract: To investigate the influence of selenium on hepatic antioxidant capacity in ducklings administrated with Afatoxin B1 (AFB1), ninety 7 days old ducklings were randomly divided into three groups (groups I-III). Group I was used as a blank control. Group II was administered with AFB1 (0.1 mg kg-1 body weight). Group III was administered with AFB1 (0.1 mg kg-1 body weight) plus selenium (sodium selenite, 1 mg kg-1 body weight). All treatments were given once daily for 21 days. The results showed that the activity of hepatic Superoxide Dismutase (SOD), Catalase (CAT), Glutathione Peroxidase (GSH-Px) and Glutathione Reductase (GR) in group II ducklings significantly decreased when compared with group I (p<0.01). Furthermore, the content of hepatic Malondialdehyde (MDA) significantly increased (p<0.01). However, the activities of hepatic SOD, CAT, GSH-Px and GR in group III ducklings significantly increased when compared with group II (p<0.05). In addition, the content of hepatic MDA significantly decreased (p<0.01). These results revealed that AFB1 significantly induced hepatic antioxidant function dysfunction. However, selenium could significantly alleviat the negative effect induced by AFB1.