Abstract: This study was conducted on 150 goats (up to 1 to >6 years) in Khoy area in the North of Iran at Spring of 2010 in order to seroprevalence of leptospiral infection. Blood samples were collected from the animals and sera were initially screened at dilution of 1:100 against 6 live serovars of Leptospira interrogans: Pomona, Canicola, Hardjo, Ballum, Icterohaemorrhagiae and Grippotyphosa using the Microscopic Agglutination Test (MAT). The serum positive goats were in 4-6 years and the seroprevalence of leptospiral infection was 13.3% in them. About 20% of infected goats were in the moist stables and 80% were in the moist (marshy) stables. There was a significant difference between the seroprevalence of disease in two kind of stables (p<0.05). Also statistically, a significant relationship observed between the increased age of examined animals and the seroprevalence of disease (p<0.05). The highest number of reactors in goats was 80% due to serovar grippotyphosa and 20% due to serovar Pomona. The majority of titer levels were 100 for all the serovars. These results confirm that the majority of leptospiral infections are asymptomatic and indicate the risk of exposure of organism to other animals.