Abstract: To clone the IL-10 promoter of rabbit and analyse its sequence, this study amplified the 5 flanking region of rabbit IL-10, compared the genomes and used bioinformatics software to make a forecast. Then, the researchers comfirmed partial promoter sequences and analysed some transcription factors of the IL-10 gene promoter. The study showed that rabbit and human IL-10 gene promoter had some similarities which all cotained GAGA, CAAT and TATA. The transcriptional start site started from -80 bp of translation initiation codon (ATG) upstream region. The promoter also had some transcription factors such as AP-1, Myogenin, c-Ets-1, C/EBP alpha and so on. Results of this study predicted partial sequences, transcriptional start site and some transcriptional regulating factors binding sites of IL-10 gene promoter which would lay the foundation of lucubrating the expression and regulation of rabbit IL-10 gene.