Abstract: An experiment was conducted to study the effects of different levels of perlite on serum calcium, phosphor and chloride in broiler chicks. The experimental design was arranged as randomized complete blocks in 4x2 factorial arrangement of treatment. About 180 male broilers of Ross 308 commercial hybrid was designated into 3 groups (0, 2 and 4%). About 3 replicates of 20 birds were assigned to each treatment. Control treatments were fed base diet and treatments with the same base diet plus 2 and 4% perlite. The feeding continued up to the age of 42 days. At the 21 and 42 days, 6 birds from each treatment were selected for serum calcium, phosphor and chloride in broiler chicks. Blood samples were collected from the vena axillaries). As intake of perlite, significantly increased serum calcium, phosphor and chloride at weeks on broiler chicks (p<0.05) these data suggested that perlite administration did not have significant effect on control treatment comparing to treatment.