Abstract: The objective of this retrospective study was to evaluate hematological and serum chemical characteristics of Open-Cervix Pyometra (OCP) and Closed-Cervix Pyometra (CCP) in bitches. In terms of hematologic characteristics, bitches with OCP had mild normocytic, normochromic anemia and less leukocytosis. With regard to serum chemistry, a disproportionate increase in Blood Urea Nitrogen (BUN) was seen in CCP (BUN:creatinine ratios >30:1). The most prominent finding was the alkaline phosphatase level in CCP (307.5 u L-1). A mildly elevated glucose concentration was seen in OCP (147.9 mg L-1). The present study provides valuable characteristics of open-cervix and closed-cervix pyometra in bitches.