Abstract: To investigate the co-infection status of Reticuloendotheliosis Virus (REV) with Chicken Anemia Virus (CAV) in chicken flocks of live poultry market, 370 spleen samples from six 817 broiler chicken flocks were collected and it’s DNA was detected by nucleic acid hybridization with the REV-specific and CAV-specific probes. Out of 370, 20 samples could be detected by REV probe and 87 samples detected by CAV probe. The positive rate of co-infection of REV with CAV was 3.2%. These results indicated that co-infection of REV and CAV also existed in healthy broiler chicken flocks in the live poultry market. A subclinical infection of REV and CAV might be an important channel for cross-infection in different chicken flocks.