Abstract: The susceptibilities of 13 recent field isolates of Mycoplasma bovis from the Ningxia province of China to 13 antimicrobial agents were detected using a microbroth dilution method. The rates of resistance to gentamicin, tilmicosin, erythromycin, lincomycin were 8/13, 7/13, 12/13 and 8/13, respectively according to the CLSI resistance breakpoint. Less than 50% of strains were resistance to ciprofloxacin, doxycycline and florfenicol with the resistance rates of 4/13 and 3/13, respectively. The lower resistance rates were observed for tiamulin (0/13), spectinomycin (1/13), enrofloxacin (2/13) and ofloxacin (2/13) suggesting the higher susceptibility of tiamulin, spectinomycin, enrofloxacin and ofloxacin to the M. bovis.