Abstract: In this study, for the first time, researchers used kinetin, a plant hormone to treat myocardial ischemia. Researchers detected the effect of kinetin on myocardial ischemia by combining in vitro and in vivo experiments in physiological and cellular levels. After analyzing the results of kinetin on ECG, cardiac physiology forms, cultivation of rat myocardial cells, NO content, activities of myocardial and antioxidant enzymes. As the result, researchers found that kinetin protected heart from myocardial ischemia by scavenging the free radical, inhibiting the lipid peroxidation and apoptosis and maintaining the structure of myocardium, myocardial cells and vascular endothelial.