Abstract: The main purpose of this study was to investigate the effects of polysaccharides from the roots of Achyranthes bidentata (ABP) on exhaustive exercise-induced oxidative stress by measuring related biochemical parameters of rats. The animals were randomly divided into four groups (n = 10 in each group): one control group and three ABP administered groups. The control group was given saline solution and the administered groups were given different doses of ABP (50, 100, 200 mg kg-1) by gavage once a day. After 21 days, the rats performed an exhaustive exercise test on a graded treadmill, along with the determination of related oxidative stress parameters. The results showed that ABP could significantly increase Superoxide Dismutase (SOD), Glutathione Peroxidase (GSH-Px) and Catalase (CAT) activities, decrease Malondialdehyde (MDA) contents in muscle of rats which meant that ABP effectively attenuates oxidative stress induced by exhaustive exercise.