Abstract: Cryptosporidiosis in animals is always with the symptom diarrhea which is not easily diagnosed and even be overlooked. Currently, researchers present here a case of this disease in Baird’s tapir with watery diarrhea in Shanghai wild zoo. The morphology identification, fecal antigen and PCR Method have been used in the detection. Moreover, all the testing assaies have shown coincidental result and species of Cryptosporidium has been ascertained as C. suis by sequencing. Therefore, the three examination methods may be useful tools for the diagnosis of cryptosporidiosis.