Abstract: Total metal accumulation and the levels of total protein and glycogen were measured in Palaemonetes pugio after exposing the animals to 0.05, 0.1 and 0.2 ppm concentrations of chromium over 1, 7 and 15 days. Metal accumulation in tisuues was measured using atomic absorbtion spectrophotometric techniques and the levels of total protein and glycogen were determined Lowry and Anthron methods, respectively. No mortality was observed under the effect of chromium at any concentrations and exposure periods tested. Total metal accumulation increased with increasing concentrations of chromium at given exposure period while total protein and glycogen levels showed a decrease on day 15th compared with day 1.