Abstract: A Gram-positive coccus isolated from a Giant Panda was identified by 16S ribosomal RNA (rRNA) gene sequencing analysis. Phylogenetic analysis showed that the organism was a member of the family Rothia. The 16S rRNA gene sequence of the organism showed 100% similarity with R. nasimurium strain CCUG 35957. Animal experiments were conducted to study the pathogenicity of R. nasimurium SCGP. This is the first reported isolation of R. nasimurium from the upper respiratory tracta of Giant Panda.