Abstract: The detoxification effects of wood vinegar were tested in this experiment. A total of 300 healthy Avian broilers at 3 days of age were selected and randomly divided into 5 groups, basal diet, basal diet with 40 μg kg-1 Aflatoxin B1 (AFB1), basal diet with 40 μg kg-1 AFB1 and 0.5% wood vinegar, basal diet with 40 μg kg-1 AFB1 and 1.0% wood vinegar, basal diet with 40 μg kg-1 AFB1 and 2.0% wood vinegar. Broilers fed with 40 μg kg-1 AFB1 showed significant decrease in marked size and average daily feed intake while feed/gain was increased. In addition, serum total protein and globin were significantly decreased. Wood vinegar could reduce or substantially eliminate the adverse effects from AFB1 on growth performance or tissue damage.