Abstract: Nicotinamide adenine dinucleotide is a drug which is important to the health of animals and humans. Nicotinamide adenine dinucleotide transporter 1 gene have been characterized to be required for nicotinamide adenine dinucleotide bioactivety. In present experiment, the complete mRNA sequence of tobacco nicotinamide adenine dinucleotide transporter 1 gene was amplified using the rapid amplification of cDNA Ends Methods. The full-length tobacco nicotinamide adenine dinucleotide transporter 1 gene mRNA was 1,588 bp containing an 948 bp open reading frame which encodes a protein of 315 amino acids. BLAST analysis revealed that tobacco nicotinamide adenine dinucleotide transporter 1 protein shares high homology with the nicotinamide adenine dinucleotide transporter 1 of Lycopersicon esculentum (93%), wine grape (86%), soybean (81%), chickpea (79%) and foxtail millet (78%). Results also showed that tobacco nicotinamide adenine dinucleotide transporter 1 gene has a closer genetic relationship with the nicotinamide adenine dinucleotide transporter 1 gene of Lycopersicon esculentum. Prediction of transmembrane helices showed that tobacco nicotinamide adenine dinucleotide transporter 1 might be a transmembrane protein. The expression profile was studied and the results indicated that tobacco nicotinamide adenine dinucleotide transporter 1 gene was highly expressed in leaf. These results established the primary foundation of using tobacco nicotinamide adenine dinucleotide as drug for animals and humans in the future.