Abstract: Researchers conducted a serological and parasitological survey of 16 free-ranging culpeo foxes (Lycalopex culpaeus) captured in several areas of central Chile from July 2009 and October 2010. Using ELISA tests, 8 animals were positive to Canine distemper virus and 1 to Canine parvovirus. Using coprological analysis (flotation and sedimentation), researchers found the first evidence of Capillaria sp., Isospora sp. and Spirometra sp. presence in culpeo fox. The tick Amblyomma tigrinum and the flea Pulex irritans were also found. This study is a first step in investigating health issues in this fox in this biodiversity hotspot of Central Chile.