Abstract: The castration of male cattle raised for beef production is a common practice in many countries to reduce management problems such as aggressive behavior, sexual activity and dark-cutting meat. To determine the effects of the castration method on body weight change and secretion of cortisol and glucose, 27 Holstein calves (30 days old) 50.7±1.3 kg initial body weight were randomly allotted to one of three treatments: untreated Control (CON); Surgical castration (SURG) and induced Cryptorchidism (CRYP). Calves were individually fed and on the day of castration, blood samples were collected via jugular venipuncture before and every 2 h after castration for 6 h for glucose, cortisol and protein concentrations. Castration method did not influence body weight change either at 2 or 4 weeks after castration. Glucose and cortisol concentration was not influenced by castration method. Serum glucose concentration was not influenced by stressor treatment. Castration method did not influence feed intake (p = 0.14) and body weight change either at 2 (p = 0.23) or at 4 weeks (p = 0.17) after castration. Cortisol concentration was not influenced by castration method (p = 0.37). Serum glucose concentration was not influenced by stressor treatment (p = 0.19). Castration method did not influence serum protein concentration (p = 0.85). These results indicate that induced cryptorchidism is an effective method of castration in growing Holstein calves for beef production.