Abstract: This study was to determine arsenic, cadmium and lead of five freshwater fish species collected from the paddy field ponds in 5 provinces of the Northeastern region, Thailand between July and December 2012. The average concentrations (on a wet weight basis) were ranges from 0.22±0.15 μg g-1 in muscle of common silver barb (Barbonymus gonionotus) to 0.78±0.25 μg g-1 in liver of striped snakehead (Channa striata) for arsenic; 0.02±0.01 μg g-1 for muscle climbing perch (Anabas testudineus) and 0.10±0.02 μg g-1 in kidney of striped snakehead (Channa striata) for cadmium and 0.30±0.09 μg g-1 in muscle climbing perch (Anabas testudineus) and 0.72±0.22 μg g-1 in liver of striped snakehead (Channa striata) for lead. Heavy metal levels found in freshwater fish samples in the present study were lower than the Thai regulatory standard.