Abstract: This study aimed to examine the levels of interleukin-33 in Gingival Crevicular Fluid (GCF) in gingival inflammation. A total of 20 subjects, 10 patients with gingivitis and 10 healthy subjects were included in this study. Periodontal status was evaluated GCF interleukin-33 levels were analyzed by ELISA. Gingivitis patients had similar GCF interleukin-33 levels to healthy group (p>0.05). The present study demonstrated that interleukin-33 in GCF of patients with gingival inflammation was similar to healthy ones.