Abstract: To elucidate the pharmaceutical mechanism about the prevention and treatment effects of Zihuangsan, so as to provide theoretical basis on animal production. The 240, 1 day old chickens were acclimatized for 6 days and then randomly divided into groups A, B, C, D, E and F, 40 birds in each group. The birds in group A, B and C were administered with three dose such as high, middle and low of Chinese Medicine Prescription-Zihuangsan. The chickens in groups D, E and F were given with the same volume of Normal Saline (NS). The birds in groups A, B, C, D and E were infected with IBDV by eye drop, group F as normal control by using NS instead of IBDV and Zihuangsan. The 3 days after IBDV infected, 0.5 mL yolk antibody per bird in group D was given intramuscularly. Result show Zihuangsan could significantly reduce the chicken’s mortality and increase the development of positive expression of CD4+, CD8+ T in immune organs such as thymus and intestinal. Zihuangsan could regulate the immune deficiency caused by IBDV. The best dosage of Zihuangsan is middle dose.