Abstract: Bovine Viral Diarrhea Virus (BVDV), a single-stranded RNA virus can cause fatal diarrhoea syndrome, respiratory problems and reproductive disorder in herd. However, there still no effective vaccine or medicines for controlling the spreading of BVDV. In this study, researchers are interested in obtaining proteins with anti-BVDV activity in ginseng. The structure protein E0 of BVDV was used as bait protein to screen the ginseng cDNA library by the yeast two hybrid assay system. The result showed that eukaryotic translation initiation factor 5A (eIF5A) of ginseng has direct interaction with E0. The interaction was verified by GST-pull down analysis. This interaction provides a target for researching of antiviral drugs for controlling the spreading of BVDV.