Abstract: To investigate the Reticuloendotheliosis virus infection status in large-scale breeder chicken farms of China, 563 serum samples from 4 great-grandparents breeder farms, 6557 serum samples from 8 grandparents breeder farms, 312 serum samples from parents breeder farms, totally 7432 serum samples were collected and examined using the Reticuloendotheliosis Virus Antibody Test kit. The results showed that REV-antibody positive rates of great-grandparents breeder flocks, grandparent breeder flocks and parents breeder flocks were 0-31, 0-47 and 4.23-42.50%, respectively. REV-antibody positive rate has close relationship with incidence of REV infection history and tumor complaints. This study suggests that REV infection is very common in China large-scale breeder chicken farms and the prevention of REV should be given more attention this is the largest scale serological survey for breeder chickens in China so far.