Abstract: Calpastatin (CAST) is a specific inhibitor of the ubiquitous calcium-dependent proteases-μ-calpain and m-calpain, found in mammalian tissues. In the coding region of the bovine CAST gene (CAST) the new nucleotide sequence polymorphism was found being a substitution G→C at position 962 nt (consensus sequence-GenBank AF281256). Consensus of bovine CAST sequence with that of human revealed that G→C substitution was located at position 1460 nt of exon 12. Computer analysis of the mutation showed the Ser→Thr substitution for CAST protein. The frequency of alleles C and G was 0.41 and 0.59, respectively. The message from these results is that CAST gene may probably be considered as a candidate marker for beef quality.