Abstract: Trypanosomiasis is a haemoprotozoan disease which affects both human and all species of domesticated mammal, especially Trypanosoma evansi in dromedaries. In the present study, early tool for prediction of Trypanosoma evansi infections related to seroprotein changes of dromedary in Tunisia was studied. Animals were distributed into 3 groups on the basis of their status related to trypanosomiasis by indirect immunoflurorescence-test: Healthy animals with negative serology for T. evansi infection, seropositive animals without clinical signs (apparently healthy) and seropositive diseased animals. Quantifiable changes in the electropherogram were apparent in dromedaries infected by trypanosomiasis with a significant hyperprotidemia and an increase of the gamma globulins fraction in seropositive animals with or without clinical signs (p<0.05). Moreover, seropositive camels that are not suffering from majors zoonotic bacterial diseases can cause immunoglobulin secretion. The results from this study showed that there was specific pattern in serum protein and electrophorerogram that could be used to distinguish infected with or without symptoms of T. evansi infection from uninfected animals. It appears that serum protein electrophoresis can be used by clinicians to predict trypanosomiasis with great ease in outbreak of T. evansi infection in combination with serological, clinical and laboratory findings of this disease.