Abstract: The present study investigates if Electroacupuncture (EA) during Hyperbaric Oxygen Treatment (HBOT) has greater effect in Traumatic Brain Injury (TBI) patients than HBO or EA alone. Thirty patients were randomized into three groups: a Hyperbaric Oxygen (HBO) group (n = 10) an EA group (n = 10) and an EA during HBO group (n = 10). Before, during and after EA treatment, variations in Transcutaneous oxygen Pressure (TcPO2) were monitored in each group. Before and after treatment, variations in Glasgow Coma Scale score (GCS) were monitored in each group. During EA treatment, the increase in TcPO2 among subjects in the EA during HBO group was significantly greater than that observed among subjects in the HBO and EA groups (p<0.01). After EA treatment, the average TcPO2 level in the EA during HBO group remained significantly higher than that observed in the other two groups (p<0.01). Moreover, the total TcPO2 level measured in the EA during HBO group was significantly higher than that of the other two groups (p<0.01). After treatment, the GCS level measured in the EA during HBO groups was significantly higher than that of the other two groups (p<0.01) and the total GCS level measured in the EA during HBO group was significantly higher than that of the other two groups (p<0.01). The therapeutic effect of EA during HBO on TBI was greater than that of HBO or EA alone.