Abstract: Proteomics is an important supplement for genomics research, every process of life is controlled by interactions between proteins. Proteomics is an objective, complicated, interlaced and precision controlled response network and is widely used in the study of animal development, physiological ecology and diseases, etc. Systems biology is used to sort and integrate these research results, furthermore explain the phenomenon of life at the protein level. This requires researchers of genetics, chemistry, biology, cell biology, engineering, mathematics, informatics and multi-disciplinary to make efforts collectively. In this study, researchers review the current status of proteomic technologies, discuss the research on proteomics in bovine and the development prospect of proteomics.