Abstract: To know the influence of the series multiepitopes from two chicken pathogens (NDV and IBDV) on their immunogenicity as well as on the boosting this immune response mediated by Ii segments (Cyt/TM) in present research, researchers linked NDV-HN (172-201) and IBDV-VP2 (197-209) in various series and further linked the both hybrids with Ii segments (Cyt/TM) and detected their immune effect in an animal experiment. The results showed that all the recombinant epitopes as single or multiepitopes with or without Ii carrier could induce mice to produce antibodies which specifically bound to VP2 (197-209) or HN (172-201) in Western blotting. The mice immunized by alone epitopes produced antibody titers only from 0.8x104-1.25x104 while the mice immunized by the single or multiepitopes with Ii segments (Cyt/TM) had antibody titers 3.1∼3.5 fold than the titers by those without the Ii segments (p<0.01). It was interesting that there was no difference in the antibody titers of groups between the single epitope and series multiepitopes with the Ii segments (p>0.05). These suggest that Ii segments (Cyt/TM) as a potential immune carrier could improve the immune response to single epitope as well as multiepitopes.