Abstract: A recombinant Herpes Virus of Turkeys (HVT) vaccine expressing the key protective antigen of Newcastle Disease Virus (NDV) could facilitate in the prevention and control of both Marek’s Disease Virus (MDV) and NDV infections. The VECTORMUNE® HVT NDV vaccine is a recombinant vaccine in which the F gene of a NDV lentogenic strain has been inserted into the HVT genome. In the current study, this vaccine was assessed for its safety in chickens. Based on clinical observations, gross pathological examinations, histopathological examinations, virus shedding and tissue distribution, the VECTORMUNE® HVT NDV vaccine was determined to possess a high level of safety in chickens.