Abstract: The presence of non-01 Vibrio cholerae in water samples and their pathogenic characteristics were studied. Three hundred (300) water samples were collected from streams and examined bacteriologically according to standard methods. Out of 300 waster samples, 36 (12%) samples were positive for non-01 Vibrio cholerae. Experimental animals were orally infected with pathogenic non-01 Vibrio cholerae isolates and the electrolytes (sodium, potassium and chloride ions) and haematological (haemoglobin, white blood cells and packed cell volume0 levels were determined. Potassium ion (K+) decreased by 3.2 mMol L 1 (42.9%) and sodium ion (Na+) decreased by 63.8 mMol L 1 (50.8%) while chloride ion (Cl-) decreased by 50.2Mol L 1 (49.6%) after 96 hours post infection. Haemoglobin (Hb), Packed Cell Volume (PCV) and White Blood Cells (WBC) values obtained in experimental animals increase! d from control value up to the seventh day and thereafter decreased. Significant differences (ANOVA, p<0.05) were observed in the electrolyte loss but not in HB, PCV and WBC values during the monitoring period.