Abstract: This study is aimed at studying the efficacy of three different solvents in the extraction of oil from Acacia Nilotica. The solvents employed are Petroleum Ether-Ethanol 50:50; Acetone and Chloroform-Methanol 50:50. The extraction on each of the solvents was done at different time interval of 2 h for five runs. The results obtained showed that the yield of oil when petroleum-ethanol (50:50) was used at 2,4,6,8 and 10 h are 1.71, 3.83, 5.82, 7.08 and 7.31%, respectively. The yield of oil obtained using acetone as solvent at the same time intervals are 5.37, 6.96, 7.71 , 8.45 and 8.24% while for chloroform-methanol (50:50), the following yield of oil was obtained 1.37, 3.83, 6.58, 7.94 and 8.33% also at the same time interval. Findings revealed that chloroform- methanol mixture is a better solvent among the three used in the extraction. The result also alluded to the usual convention of extraction of oil increase with time.