Abstract: This research is an investigation of the application of liquid smoke produced from the pyrolysis of Palm Kernel Shell (PKS) controlling anthracnose, a fungal disease caused by Colletotrichum capsici on chili. The experiments for liquid smoke production were carried out in a batch reactor apparatus on a range temperature of 100-350°C. The results showed that pyrolysis temperature and liquid smoke concentration significantly influenced the fungus spot caused by C. capsici. A chemical compound present in liquid smoke in treatment T2 was able to impair a growth of the fungal mycelium of C. capsici. The smallest spot diameter was found at concentration K3 with an average value of 0.454 cm; although it was not significantly different from the sizes of treatment K2 = 0.504 cm and K5 = 0.519 cm. However, the best treatment was obtained from a combination of treatment T2K4 (Pyrolysis temperature of 200-250°C and liquid smoke concentration of 8%). A long period of incubation in treatment of T2K4 and 4.5 day after incubation showed that there was a resistance characteristic in the treatment which caused the disease symptoms to appear in long time, making the pathogens to evolve longer.