Abstract: It is to analyze antioxidant and antibacterial activities of several solvent extracts from Hypsizygus marmoreus. Antioxidant activity was evaluated by determining DPPH/ABTS activities, TPC and TFC. In addition, the antibacterial activity was determined against six bacterial pathogensby disc diffusion method. DPPH radical scavenging activity (93.3±0.10%) and ABTS radical scavenging activity (97.2±1.22) were significantly high in methanol extracts which also showed higher value of TPC and TFC as compared other extracts. In addition, all extracts showed an inhibition effect against P. aeruginosa and one of them, the ethyl acetate extractsespecially showed a very high inhibition effect against Gram (-) bacteria (Ent. cloacae, E. coli and P. aeruginosa). Hypsizygus marmoreus has a high biological active effect related to antioxidant and antibacterial activity and so their utility value is so high in various health foods or functional cosmetic products.