Abstract: Mobile banking (m-Banking) application is one of the emerging trends of mobile commerce application. m-Banking application allowed customers to perform their transactions at their own convenience time. Usability issues is one of the major problems faced by m-Banking applications and there is less research on comparing usability issues based on user’s age, gender, qualification and experience. The objectives of this study are to identify usability issues and levels of differences among the m-Banking users based on their age, gender, qualification and experience. This study employed quantitative approach and has obtained one hundred and fifty respondents from three banks in Nigeria. The finding of this study indicates that the banks have usability issues on privacy and reliability of their m-Banking application interfaces. This study also revealed that male users have less usability issues than female users, younger and middle age users also have few usability issues than the old age users. For qualification of the users with lower qualification, they have more usability issues than those that have higher qualification. While in years of experience using the mobile, the result shows that there are not much usability issues for the whole category of years.