Abstract: Microfabrication technique based on microelectronic technology has the capability to produce microfluidic devices in micro or nano-meteric scale but this technique involves with costly process and toxic chemicals. In this study, we proposed the use of patterned vinyl adhesive template to produce a flow focusing microfluidic device on a work bench that was applied to generate microbeads of calcium alginate for microencapsulation of cells. In this researche an infusion pump of high flow rates (1000-5000 μL/min) was developed to be used together with a commercial syringe pump that could pump the fluid to emulsify the continuous and disperse phases leading to the production of microbeads. Microbeads of calcium alginate in the range of 200-800 μm was successfully produced using this simple, environment friendly and efficient technique.