Abstract: Mobile Ad hoc Networks (MANETs) is a wireless technology that plays an important role in several modern applications which include military, civil, health and real-time applications. Providing Quality of Service (QoS) for this application with network characterized by node mobility, infrastructure-less, limitation resource is a critical issue and takes greater attention. However, transport protocols effected influential on the performance of MANET application. This study provides an analysis and evaluation of the performance for TFRC, UDP and TCP transport protocols in MANET environment. In order to achieve high accuracy results, the three transport protocols are implemented and simulated with four different network topology which are 5, 10, 30 and 50 nodes, respectively using well known Network Simulator (NS-2.35). Moreover, Constant Bit Rate (CBR) considered as a traffic source and On-demand Distance Vector (AODV) as the routing protocol. For evaluation performance, QoS metrics such as end-to-end delay, packet delivery ratio, throughput and jitter are measured. The results show that delay and jitter of TFRC are slightly less than UDP and TCP whereas UDP has the significantly better performance wise throughput.