Abstract: Composite films of Poly (Methyl Methacrylate) (PMMA)/SiO2-ZnO-Ch are prepared by casting method and evaluated for medical application. Nano silica was prepared from water glass by precipitation method, chlorophyll (Bio natural pigment) extraction from medicago sativa and use as plasticizer. In this study, nano silica and nano zinc oxide are used as filler to improve the biological and mechanical properties of composite films. Where nano silica has good mechanical properties and nano zinc oxide has excellent antibacterial activity (SiO2-ZnO and Ch) are used with a following percentages (1-3) wt.% to the polymer. Many tests are carried on composite films such as antibacterial activity, Fourier Transform Infrared spectroscopy (FTIR) and tensile test. The result shown that zinc oxide has excellent antibacterial activity. And its activity in composite films increasing with increasing its concentration.