Abstract: Computational approaches have been widely used to study both physical and life phenomena beyond the traditional purview of computer science. In this study, web services and computer algorithms were used to study the binding ligands of Interleukin-5 (IL-5) which is one of the critical cytokines in the immune system. Ramachandran analysis with visual plot was carried out. The structural perspective of IL-5 ligand binding was studied to determine the outliers. This study contributes to the understanding of the structural binding of IL-5 ligands that may lead to a complete picture of signaling pathways in the immunological context.