Abstract: In this study, we describe the experimental material on the study of the expediency of application of aeroionization in different livestock enterprises. It has been established that in the air of livestock houses the content of light negative ions is 5.6 times less than in the atmosphere and of heavy ones, on the contrary, it is 1.8 times more. The ion pollution coefficient is 10 times higher than in the atmosphere. The use of aeroionization increases the sanitary condition of the microclimate with the relative humidity reduced by 6.9-8.1%, carbon dioxide content by 0.03%, ammonia by 2.0 mg/m3, hydrogen sulfide by 1.0 mg/m3, the amount of dust and germs by 1.8 times. Under the influence of optimal concentrations of light negative ions, hematological factors, the level of natural resistance of animals, average daily live weight gain of piglets are improved by 13.1%, calves by 13.9% in relation to control animals. Combined application of aeroionization, “Erakond”, “Biostim” tissue preparations and “Sporovit” and “Lactobacterin” probiotics has a more significant stimulating effect on hematological factors, the level of natural resistance and the intensity of growth of animals than their separate application. Thus, the average daily gain increased by 15.8%, the indices of natural resistance improved: lysozyme activity of blood cell increased by 5.9%, bactericidal activity by 5.9%, phagocytic activity by 7.0%, complementary by 9.0%. Combined application of aeroionization, biological stimulants and probiotics is not only hygienically expedient but it is a highly effective measure with the pay back of 8.85/1 rub. of expenses.