Abstract: People’s health and wellbeing depend on the quality of healthcare services provided by healthcare organizations. This study aimed to investigate the determinants of the quality of healthcare services provided in Nigerian healthcare organization. The study was based on descriptive research design. The sample for the study consisted of 650 healthcare stakeholders selected from the 36 states of Nigeria. The 36 states constituted 36 clusters (cluster sampling techniques). The simple random method (balloting) was used to select 10 states out of the 36 clusters. A total of 65 healthcare stakeholders were chosen from each of the 10 states (clusters), using the simple random method of sampling. Overall, a total of 650 healthcare stakeholders were used for the study. The study showed the following results: the majority of the healthcare stakeholders were females (58.5%); between the ages of 31-50 years (41.5%); Possessed a degree as their highest educational qualifications (51%) were married (60.5%) and were healthcare providers (32.3%). Available data indicated that the determinants of the quality of healthcare services provision in Nigerian healthcare organizations included provider-related factors (91.0%), organizational-related factors (84.6%) and patient-related factors (63.4%). The quality of healthcare services provision in Nigerian healthcare organizations is determined by provider-related factors, organization-related factors and patient-related factors, respectively. The Nigerian health care system should support quality improvement activities, effective leadership and partnership development to ensure sustainability and effectiveness in the provision of quality healthcare services.