Shawket Kadhim Jawad, Musa Umran Kadhium and Ebaa Adnan Azooz

Abstract: By using two approaches cloud point extraction technology CPE and liquid ion exchange to separate and extracted Fe(III) and Hg(II) ions from acidic HCl media as ion pair association complexes by using new laboratory prepared 2-(4-hydroxy phenyl azo)-4-benzene naphthol (HPBN) whereas the spectrophotometric studies show the new organic reagent (HPBN) have a wave length for maximum absorbance was λ_max = 450 nm, the ion pair association complex of Fe³⁺ λ_max= 477 nm but for Hg²⁺ λ_max= 476 nm, so that, the optimum condition for high extraction efficiency was 0.5M HCl for both ions ,100 μg metal ions in 10 mL aqueous solution 1×10^-4 M of HPBN and necessary to heating the solution at 90°C for 20 min to Fe³⁺ ion and 15 min. For Hg²⁺ ion and in presence of 0.5 mL Triton X-100 for Fe³⁺ and 0.8 mL Triton X-100 for Hg²⁺ ion. As well as the research involved other studies such as electrolyte effect interferences effect, stoichiometry, application and determination of trace amounts of Fe(III) and Hg(II) in environmental sample by spectrophotometry.