Abstract: Mobile Ad hoc Network (MANET) faces significant problem and challenges in multiple path routing over wireless communication compared to the wired network. Routing is one of the important protocol and acts as a major connection between the nodes for providing reliable and effective communication in MANET. It is hard to implement a secure routing protocol for various attacks due to its several potential MANET security threats and network environments. A bio-inspired routing protocol was used in this study by incorporating swam based approach for an identification of malicious attack. A multi-agent immune system is used by accompanying Intrusion Detection System (IDS) in order to reduce the false alarm rate successfully. Moreover, in this study, we find the shortest distance and provide security between the nodes source and destination using Ant Colony Optimization (ACO). As a result, the proposed SAMR (Secured Ant-Based MANET Routing) method is compared with some existing technique based on throughput, control packet overhead, end to end delay and packet delivery ratioto show better performance analysis, respectively.