Abstract: In this study, the absorption and fluorescence spectra of (Azure-A and B) dyes dissolved in ethanol and different concentrations were studied. The optical properties (absorbance, transmittance, linear absorption and linear reflective index) were calculated as well as the calculation the lifetime and quantum efficiency of fluorescence. The results shows the increasing of absorption values and decreasing of transmittance values when the concentration increase, this is accordance with Lambert’s law. Also, the results shows increasing the values of the lifetime of fluorescence and decreasing the quantum efficiency.