Abstract: Powder of dried of N. palea and N. incerta were mixed together in equal ratio and used as different grams 0.2, 0.4, 0.6, 0.8 and 1 g were added to the selected heavy metals Ni, Cd and Pb in concentrations 0.5, 1, 2 ppm which recorded after 1, 4 and 6 h to determined the contact time of high percentage removal. The results showed the optimum removal percentage for cadmium was in pH 8 with room temperature 25±2°C. High decreased in cadmium ions with a range between 0.003-0.04 mg/L for the concentration 0.5 ppm with percentage removal reached 90.7-99.4%. Biosorption of lead indicated high removing in the concentrations 2 ppm with percentage removal 91.5-96.6%. As well as for nickel, the data showed the high decrease in the concentration 2 ppm with percentage removal reached 93-96.5%. FTIR technology was depended for detecting the active groups on cells surfaces for the two diatoms which responsible for biosorption of lead, nickel and cadmium.