Abstract: Different salts NaCl, CaCl2.2H2O, MgSO4.7H2O and their combination, NaCl + CaCl2 + MgSO4.7H2O were employed to establish different equimolar strengths i.e. 50mM, 100mM, 150mM and 200mM to study their effect on Brassica Juncea shoot regeneration from leaf explant. Best shoot regeneration in percentage (80%), number (8), length (10mm) was recorded in 50mM of NaCl+MgSO4.7H2O.+CaCl2. 2H2O. CaCl2 proved to be more deleterious in comparable concentration as that of NaCl and MgSO4.7H2O. No age and position factor was observed for shoot regeneration however it was comparatively more towards lower halves of leaf lamina.