Abstract: Studies were carried out on preparation of a medium from an infusion of sweet potato for culturing of yeasts from suitable suspensions of pure culture. Growth of yeasts on the sweet potato agar (SPA) was comparable and sometimes higher than those obtained on potato dextrose agar (PDA). Supplementary nutrients namely, 0.3% yeast extract, 2.0, 1.0 and 0.5% glucose did enhance the performance as a good substitute for imported yeast isolation media. The yeast species used as test organisms were Candida albican, Geotrichum candidum, Rhodotorula sp., Saccharomyces cerevisiae Baker`s yeast and Saccharomyces cerevisiae Brewer`s yeast.