Abstract: The present study is concerned with the production of amyloglucosidase by UV irradiated strain of Aspergillus niger. The parental strain of Aspergillus niger was subjected to UV irriaditions for 5-30 minutes. One hundred and ten mutants of Aspergillus niger were isolated by observing the hydrolysis of starch in the petriplates. Of all the mutants tested, the mutant strain of Aspergillus niger GCBU-25, isolated after 25 minutes of UV irradiation gave the maximum production of AMG (136.1 IU/ml/min), which was two fold increase in the production of the enzyme than the parental strain. The production of AMG reached maximum (183 IU/ml/min) when starch at 1% level and (NH4)2SO4 at 0.2% level of nitrogen was used as carbon and nitrogen source respectively.