Abstract: The objective of this study was to investigate, the changes in physicochemical characteristics of date fruit (Phoenix dactyliferaL.) cultivars at Kimri, Khalal, Rutab and Tamar state by two different propagation methods. Weight and length of whole fruit and seeds were significantly higher in vitro cultivars (16.74±0.38, 4343±0.15) as compare to offshoot cultivars (1.53±0.02, 2.93±0.15), could be the result of older productivity. Ash (1.77±0.44), protein (3.23±0.08) and Carbohydrate (8.48±0.49) were recorded maximum at Tamar stage. Significant amount of fructose and glucose were calculated higher at Tamar stage from in vitro cultivars. Higher percentage of Potassium (419.64±12.12) and Iron (2.3±0.04) among micro nutrients were detected in date fruits, which is considered a front contributor in Saudi Arabian diet.