Abstract: In this study, we proposed a novel method to milk sterilization based on laser technology, the design method was practically implemented and tested under different operating conditions. The milk treatment sample with LD laser (650 nm) was investigated. Inactivation of different microbial can be achieved at process time (0.5-2) min at laser power level (50-70 mw), the study showed that the maximum microbial reduction (100%) was obtained at 1.5 min with 70 mw. There was no significant variation in physical chemical properties of treatment sample in comparing with control milk sample.