Abstract: Three cultivars of loquat including Longquan1, Dawuxing, Zaozhong6 were used to study the effects of different pre-treatments on callus induction from anther. The results showed that there were no anther callus induction without any pre-treatment (CK), the anther callus induction rate of bud with pre-treatments was significantly increased. In all the pre-treatments, low temperature (4°C) was the best for anther callus induction and followed by heat shock, high osmotic pressure and centrifugation; 48 h low temperature (4°C) pre-treatment was beneficial as it significantly increased the induction rate of callus and callus induction rate changed with cultivars, reaching the apex of 54.67, 46.33 and 72.00% for cv. Longquan1, Dawuxing and Zaozhong6, respectively.